High-Specific Electrochemical Detection of BRCA1 GeneSequence using Methylene Blue Hybridization Indicator

A high-specific and high-sensitive electrochemical DNA biosensor for human breast cancer susceptibility gene 1 (BRCA1) was developed by using methylene blue (MB) as hybridization indicator. A specially designed capture probe with a thiol group at its 5’-end was firstly self-assembled on gold electrode through Au-S bond. In the solution with high ionic strength, MB combined with the capture probe through its interaction with guanine.